nucleasep1

Nuclease P1 is used to improve the sensitivity of a 32P-labeling method for the detection of DNA adducts. The enzyme has an optimal temperature of approximately ...

Nuclease P1 is a zinc-dependent single-strand specific nuclease which hydrolyzes 3'->5' phosphodiester bonds in RNA and ssDNA.

由 JD Jones 著作 · 2023 · 被引用 6 次 — Nuclease P1 is an endonuclease with no nucleobase specificity that is commonly used in a two-step enzymatic process to digest RNA down to ...

Nuclease P1 from Penicillium citrinum lyophilized powder, >=200 units/mg protein (E1%/280, 3-5-Phosphodiesterase). NT$35,900. 品牌:SIGMA 品號:N8630

Nuclease P1 is one of the most well-known single stranded specific nucleases in the field of molecular biology, it is widely used in the pharmaceutical and food ...

One unit of nuclease P1 is added per 24 mg total nucleic acid. Digestion is carried out at pH 8.0,55°, for 2 hr, and the nucleic acids are again denatured and ...

由 C Romier 著作 · 1998 · 被引用 144 次 — Recognition of single-stranded DNA by nuclease P1: high resolution crystal structures of complexes with substrate analogs. Proteins. 1998 Sep 1;32(4):414-24.

由 M Silberklang 著作 · 1977 · 被引用 276 次 — The method involves partial endonucleolytic digestion of the labeled RNA with nuclease P1 (from Penicillium citrinum) followed by separation of the partial ...

核酸酶P1 是一种锌依赖的单链特异性核酸酶，可水解RNA 和单链DNA（ssDNA）中的3´→5´ 磷酸二酯键。